Effect of Yang-warming and Qi-tonifying Recipe on Colonic Aquaporin 3,8 Expression and Intestinal Motility of Loperamide-induced Constipation Rats
10.13359/j.cnki.gzxbtcm.2018.01.024
- VernacularTitle:温阳益气方对便秘模型大鼠结肠水通道蛋白3、8表达及肠道动力的影响
- Author:
Sheng Ben WU
1
;
Qing ZHOU
;
Shuai YAN
;
Ming Ming SUN
;
Hui Ying CHEN
;
Peng Xiao WANG
Author Information
1. 苏州市中医医院
- Keywords:
slow transit constipation;
Yang-warming and Qi-tonifying Recipe;
aquaporin 3;
aquaporin 8;
disease models,animal;
rats
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2018;35(1):112-117
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Yang-warming and Qi-tonifying Recipe (YQP)on aquaporin 3(AQP3) and AQP8 in rats with slow transit constipation,and to explore its therapeutic mechanism. Methods Forty rats were randomly divided into modeling group(N=30)and normal control group(N = 10). After successful modeling by gastric gavage of loperamide,the modeled rats were randomly divided into model group,YQP group and Mosapride group,10 rats in each group,and were separately treated with corresponding medicine for 2 weeks. After treatment, the colonic transit function was measured by carbon propelling test. The protein levels of AQP3 and AQP8 were detected by immunohistochemistry and their mRNA expression levels were detected with real-time fluorescence polymerase chain reaction (qPCR). Results Compared with the normal control group,the propelling rate of carbon particle in the model group was decreased,and the protein and mRNA expression levels of AQP3 and AQP8 were significantly increased(P<0.05 or P<0.01). Compared with the model group,the propelling rate of carbon particle of YQP group and mosapride group was significantly increased, and the protein and mRNA expression levels of AQP3 and AQP8 were significantly decreased (P<0.05),but there was no significant difference between YQP group and mosapride group (P >0.05). Conclusion YQP had therapeutic effects on loperamide-induced constipation through decreasing the expression of AQP3 and AQP8 in the intestine,reducing the reabsorption of intestinal fluid, and increasing the fecal water content.
