Effect of Kidney-nourishing,Blood-activating,Phlegm-resolving and Resuscitation-inducing Decoction on Expression of NMDA Receptor Subtypes in Cochlear Spiral Ganglion Neurons of Tinnitus Rats
10.13359/j.cnki.gzxbtcm.2018.01.027
- VernacularTitle:补肾活血化痰开窍方对耳鸣大鼠耳蜗螺旋神经节神经元NMDA受体亚型表达的影响
- Author:
Guo Qin SUN
1
;
Meng LUO
;
Bo JIANG
;
Mei Xian ZHANG
;
Jie Hong XU
;
Jie Yan MOU
;
Kun LYU
;
Qian YANG
Author Information
1. 武汉大学附属同仁医院/武汉市第三医院
- Keywords:
Kidney-nourishing,Blood-activating,Phlegm-resolving and Resuscitation-inducing Decoction;
cochlear spiral ganglion neurons;
NMDA;
NR1;
NR2A;
NR2B;
tinnitus;
disease models,animal;
rats
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2018;35(1):129-134
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Kidney-nourishing, Blood-activating, Phlegm-resolving and Resuscitation-inducing Decoction(KBPRD)on the expression of N-methyl-D-aspartate(NMDA)receptor subtype 1,2A,2B(NR1,NR2A,NR2B)in the cochlear spiral ganglion neurons(SGN)of tinnitus rats and to explore its mechanism, thus to provide experimental evidence for the treatment of tinnitus with KBPRD. Methods Sixty rats were randomly divided into normal group,model group,western medicine group,and low-,middle-,and high-dose Chinese medicine groups, 10 rats in each group. Rats were given intraperitoneal injection of sodium salicylate combined with water deprivation to induce tinnitus model. After successful establishment of the model, the rats in low-,middle-,and high-dose Chinese medicine groups were given gastric administration of KBPRD in the dosage of 5.5, 11, 22 g·kg-1·d-1 respectively, the rats in western medicine group were given gastric administration of 5 mg·kg-1·d-1 of carbamazepine,and rats in the model group and normal group were given gastric administration of 2 mL of normal saline,once every day,treatment time covering 8 weeks. The expression levels of NR1,NR2A,and NR2B in the cochlear SGN was detected by immunoblotting and real-time quantitative reverse transcription-polymerase chain reaction(qRT-PCR)after 8 weeks of treatment. Results Compared with the normal group,the expression levels of NR1, NR2A and NR2B in the model group were increased, the difference being significant (P < 0.05). Compared with the model group,the expression of NR1,NR2A and NR2B in low-,middle-,and high-dose Chinese medicine groups were significantly decreased(P<0.05).Conclusion KBPRD is effective on relieving tinnitus of rats, and its mechanism is correlated with lowering the increased expression of NR1,NR2A and NR2B in SGN of tinnitus rats.
