In vitro properties of magnesium doped hydroxyapatite coating on the surface of carbon/carbon composites
10.3969/j.issn.2095-4344.2017.34.006
- VernacularTitle:碳/碳复合材料表面掺镁羟基磷灰石生物涂层的体外性能
- Author:
ye Xin NI
1
;
bo Xin XIONG
;
jin Rui YOU
;
sheng Xing WU
;
shuai Wan LI
Author Information
1. 南京医科大学附属常州第二人民医院
- From:
Chinese Journal of Tissue Engineering Research
2017;21(34):5443-5448
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: In the early study, hydroxyapatite coatings containing magnesium (Mg-HA) were successfully prepared on the carbon/carbon composite surface by electromagnetic induction deposition. The effects of different induction deposition time on the morphology of Mg-HA coating were analyzed. OBJECTIVE:To investigate the in vitro properties of Mg-HA coating on the carbon/carbon composites. METHODS: HA (control group) and Mg-HA (experimental group) coating were prepared on the surface of carbon/carbon composites.(1)In vitro solubility:Carbon/carbon composites with different coatings were immersed in low-glucose DMEM medium, and surface morphology, ion concentration, and coating adhesion were tested before and after immersion. (2) Cytocompatibility: Carbon/carbon composites with different coatings were respectively cultured with mouse bone marrow mesenchymal stem cells for 6 days, and cell proliferation was measured by scanning electron microscope. RESULTS AND CONCLUSION: (1) One day after soaking, sediments formed on the coating surface in both groups, but there were more sediments in the experimental group than the control group. Six days after soaking, the sediments completely covered the coating surface in the experimental group, while there were still some gaps in the control group; Ca and P were both detected on the coating surface in the two groups, and the Mg content on the coating surface was obviously higher in the experimental group than the control group. (2) Six days after soaking, the concentrations of Ca, P and Mg in the control group continued to fall; in the experimental group, the concentrations of Ca and P were also reduced gradually, but the Mg concentration arose gradually. Moreover, the Mg concentration was significantly higher in the experimental group than the control group (P < 0.05). (3) The bonding strength of the coating in the experimental group was higher than that in the control group before and after 6 days soaking. In summary, the Mg-HA coating has better bonding strength and cell compatibility.
