Effect of blocking RAGE on macrophage infiltration of diabetic wound healing
10.3969/j.issn.1674-8115.2017.12.002
- VernacularTitle:外源性阻断RAGE效应对糖尿病小鼠创面中巨噬细胞浸润的影响
- Author:
Qi WANG
1
;
zan Xiao CAO
;
ya Guan ZHU
;
Fei SONG
;
liang Shu LU
;
wen Yi NIU
Author Information
1. 上海交通大学 医学院附属第九人民医院急诊科
- Keywords:
diabetes mellitus;
macrophage;
receptor for advanced glycation end products;
wound healing
- From:
Journal of Shanghai Jiaotong University(Medical Science)
2017;37(12):1588-1593
- CountryChina
- Language:Chinese
-
Abstract:
Objective·To investigate the influence of blocking receptor for advanced glycation end products (RAGE) on macrophages infiltration in diabetic wound healing. Methods·Ninety-six male C57BL/6J mice (8-week-old) were divided into diabetic group (n=72) and normal group (n=24) randomly. Diabetic mice were induced by streptozocin multiple intraperitoneal injection. One full-thickness excisional wound (diameter of 9 mm) was created by a sterilized punch. Diabetic mice were divided into 3 groups in which different topical treatments were applied to the wounds. Anti-RAGE antibody were applied in group R, rabbit IgG applied in group I, normal saline applied in group C. Normal mice were applied with saline topically (group N). All treatments were repeated on day 3 and day 7 after wounded. The wound healing conditions were observed. The wound and surrounding tissues from animals in each group were excised on day 1, 3, and 7 after wounded. Immunohistochemistry was utilized to investigate the changes of macrophages infiltration in quantity. Macrophages were also analyzed with respect to morphology by transmission electron microscopy (TEM). Results·① The wound closure ratio of group R was higher than those of group C and group I on day 14 after being wounded (P=0.000). ② On day 1, the numbers of macrophages in group R and group N were both bigger than those of group C and group I, but smaller on day 14 (P=0.000). ③ The morphological characters of macrophages also existed great differences under TEM. Conclusion·Number and morphology of macrophages are both abnormal in diabetic wound in a RAGE pathway depending manner. Based on macrophages, it suggests that impaired healing of diabetic wound is closely related to RAGE pathway.
