Effects of insulin-like peptide 6 on renal fibrosis and calcification in unilateral ureteral obstruction mice
10.3969/j.issn.1674-8115.2017.12.006
- VernacularTitle:胰岛素样因子6对单侧输尿管梗阻小鼠肾脏纤维化和钙化的影响
- Author:
Lei GAO
1
;
yong Shi WU
;
Dan JIANG
;
Ying WANG
;
yong Yong LI
;
wei De WANG
;
lei Guang CHANG
;
Peng ZHANG
;
Zheng QIN
;
ying Dong ZHANG
Author Information
1. 重庆医科大学附属第一医院 心血管内科
- Keywords:
renal fibrosis;
renal calcification;
unilateral ureteral obstruction;
insulin-like peptide 6
- From:
Journal of Shanghai Jiaotong University(Medical Science)
2017;37(12):1616-1622
- CountryChina
- Language:Chinese
-
Abstract:
Objective·To investigate the effects of insulin-like peptide 6 (Insl6) on renal fibrosis and calcification in unilateral ureteral obstruction (UUO) mice. Methods·Twenty-four SPF male mice with genotypic background of C57BL/6 were divided into Sham (n=8), UUO+saline (n=8) and UUO+Insl6 (n=8) groups randomly. Mice were sacrificed 10 days after operation and renal tissues of surgical side were obtained. Sirus red staining, Masson staining and alizarin red S staining were used to verify the level of collagen and calcium deposition. TGF-β1 expression was determined by Western blotting. Realtime-PCR was used for determining TGF-β1, BMP2, Col1a1, and Col2a1 mRNA expression. Results·Compared with sham group, fibrotic area especially collagen Ⅰ , calcium deposition, TGF-β1 protein, and TGF-β1, BMP2, Col1a1, and Col2a1 mRNA expression in UUO+saline group significantly increased (all P<0.05). As compared with UUO+saline group, fibrotic area especially collagen Ⅰ, calcium deposition, TGF-β1 protein, and TGF-β1, BMP2, Col1a1, and Col2a1 mRNA expression in UUO+Insl6 group significantly decreased (all P<0.05). Conclusion·Insl6 inhibits UUO-induced renal fibrosis and calcification, which may be related to regulation of TGF-β1, collagen Ⅰ , BMP2 and collagen Ⅱ expression levels.
