Preliminary study of flavonoids affecting cAMP level in cannabinoid receptor overexpressed cells
10.3969/j.issn.1674-8115.2017.12.007
- VernacularTitle:黄酮类化合物影响大麻素受体过表达细胞中cAMP含量的初步研究
- Author:
Ting LI
1
;
hua Jian LIU
Author Information
1. 上海交通大学 基础医学院化学教研室
- Keywords:
cannabinoid receptor;
flavonoids/flavonoid compounds;
cannabinoid receptor overexpressed cell;
cyclic adenosine monophosphate
- From:
Journal of Shanghai Jiaotong University(Medical Science)
2017;37(12):1623-1629
- CountryChina
- Language:Chinese
-
Abstract:
Objective·To evaluate the effects of flavonoids on cyclic adenosine monophosphate (cAMP) level in cannabinoid receptor (CBR) overexpressed cells. Methods·Eukaryotic expression vectors GV144-CB1R and GV144-CB2R were constructed, and transfected into HEK-293 cells to establish CBR overexpressed cell lines. Several flavonoid compounds were studied by measuring the change of cAMP level in CBR-overpressed HEK-293 cell lines. Results·The CBR overexpressed cell lines were constructed successfully. cAMP level in CBR-overexpressed HEK-293 cells decreased after incubation with flavonoid compounds. And these effects were selective for CB2R overexpressed cells. Conclusion·All flavonoids studied exhibited decreasing effect on cAMP level in CBR-overexpressed cells at μmol/L level.
