Improved treatment of frozen red blood cells by red blood cell processing technology
10.7644/j.issn.1674-9960.2017.09.006
- VernacularTitle:冰冻红细胞洗涤技术优化及应用效果评价研究
- Author:
xia Min LIU
1
;
yang Xiao YI
;
xi Jie WANG
;
Tao GU
;
Wei DU
;
wei Jian ZHOU
;
Yan WANG
;
yun Jian WANG
;
Hui CAO
;
Dong-gen YING
;
WANG HAN
Author Information
1. 军事医学科学院野战输血研究所
- Keywords:
frozen RBC;
blood cell washing instrument;
program optimization;
quality evaluation
- From:Military Medical Sciences
2017;41(9):727-729,738
- CountryChina
- Language:Chinese
-
Abstract:
Objective To improve the buffer for washing frozen red blood cells(RBCs)and explore the feasibility of replacing the middle buffer of 2% NaCl with 0.9% NaCl solution in the protocol of deglycerolization and the effect was evaluated.Methods Two units of RBCs separated from whole blood of healthy donors were frozen at -80℃.The thawed frozen RBCs were treated with a washing machine.On the basis of the old protocol(protocol 1),the middle buffer of 2%NaCl was replaced,and the usage of washing buffer and the washing steps were adjusted to create a new protocol(protocol 2).The quality of RBCs washed by the two protocols was evaluated.Results According to the results of the detection of RBCs treated by protocol 1 and 2: the hemoglobin contents(g)were 42.18 ±3.35 and 44.98 ±1.68,respectively,free hemoglobin contents(g/L)were 0.53 ±0.06 and 0.45 ±0.05 respectively, the residual amount of white blood cells (×107)was 1.92 ±1.04 and 1.12 ±1.12,and the osmolarities(mOsm)were 327.0 ±9.06 and 331.8 ±10.62 respectively. Sterile experiments were negative for both bacteria and fungi.The hemolysis rates(%)were 12.02 ±5.78 and 14.30 ± 5.67 respectively.The deforming abilities(%)were 21.42 ±1.45 and 21.32 ±0.84 respectively.The RBC recoveries (%)were 77.18 ±5.58 and 79.63 ±2.06 respectively.The processing time(min)was 79.60 ±0.55 and 78.80 ±1.30 respectively.There was no significant difference in the quality of frozen RBCs washed by the old protocol(protocol 1)and the new protocol(protocol 2)(P>0.05).Conclusion The quality of frozen RBCs washed by the two protocols meet national standards.