The effect of autophagy regulation on apopstosis of spinal cell in diabetic neuropathic pain rats
10.3969/j.issn.1006-5725.2017.24.009
- VernacularTitle:糖尿病疼痛大鼠脊髓细胞自噬与凋亡相互作用的研究
- Author:
Xiang LI
1
;
Fei HUANG
;
Nan CHENG
;
Zhuolin QIU
;
Ning SHEN
Author Information
1. 中山大学附属第三医院麻醉科
- Keywords:
neuropathic pain;
autophagy;
apoptosis;
crosstalk
- From:
The Journal of Practical Medicine
2017;33(24):4057-4060
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of autophagy regulation on apoptosis of spinal cord cell in diabetic neuropathic pain(DNP)rats.Methods The SD rats were intraperitoneally injected with Streptozotocin to establish DNP model,and were randomly divided into 4 groups:DNP group,DNP-Rapamycin(DNP-R)group, DNP-3-M(DNP-M)group and the control(C)group. Rapamycin or 3-MA was intrathecally injected in rats in DNP-R group and DNP-M group.The protein expression of LC3-II,Bcl-2 and Caspase-3 was detected by Western blot assay,the cell apoptosis was detected by TUNEL assay. Results Compared with DNP group,the expression of LC3-II was significantly up-regulated,while the expression of Caspase-3 and Bcl-2 were significantly down-regu-lated in DNP-R group. For DNP-M group,the expression of LC3-II was significantly down-regulated,while the expression of Caspase-3 and Bcl-2 were significantly up-regulated. The cell apoptosis was significantly reduced in DNP-R group,and significantly increased in DNP-M group. Conclusion There might be a crosstalk between autophagy and apoptosis in spinal cord DNP model. The activation of autophagy might inhibit the apoptosis,while the depression of autophagy might increase the apoptosis of spinal cord cells.
