Gene detection and multilocus sequence typing of carbapenem resistant Klebsiella pneumonia
10.3969/j.issn.1006-5725.2017.24.034
- VernacularTitle:耐碳青霉烯类肺炎克雷伯菌基因检测及多位点序列分析
- Author:
Liang JIN
1
;
Qi WANG
;
Shuyi WANG
Author Information
1. 066000,秦皇岛市第一医院检验一科
- Keywords:
Klebsiella pneumoniae;
carbapenemases;
resistance mechanism;
multilocus sequence typing
- From:
The Journal of Practical Medicine
2017;33(24):4156-4160
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the cause of high isolation rate of carbapenem resistant Klebsiella pneumoniae(CRKP)in the first half of 2016 and the mechanism of its resistance and homology,in order to pro-vide basis for the control of nosocomial infection.Methods We collected CRKP from January to June 2016 to con-firm carbapenemase phenotype by modified Hodge test. Carbapenemase genes were amplified by using PCR meth-od,and the PCR product was sequencing to determine the genotypes by multilocus sequence analysis(MLST)to analyze the homology.Results From January 2016 to June,89 non redundant KPN89 strains were isolated, with 41 strains of CRKP and the isolation rate was 46.07%.CRKP was mainly isolated from Department of Critical Care Medicine,Department of Neurosurgery,Department of Respiration and so on. The specimens were sputum, blood,urine,cerebrospinal fluid specimens. Forty-one strains of CRKP were 100% sensitive to polymyxin, 95.12% sensitive to complement and 4.88% sensitive to minocycline,and were resistant to the other 23 antimicrobi-al agents. Hodge test of 41 strains of CRKP was positive and sequencing confirmed that all carried KPC-2 type of carbapenems.MLST classification results belonged to type ST11. Conclusions CRKP outbroken in our hospital is MLST typing of type sT11. With serious drug resistance,it may be the same strain spread among different pa-tients in different wards and the main drug resistance mechanism is producing type KPC-2 carbapenems. All rele-vant departments should carry out epidemiological investigations timely for the prevention and control.
