Molecular Mechanism of Luteolin-regulated Epithelial Mesenchymal Transition in Non-small Cell Lung Cancer
10.3870/j.issn.1004-0781.2018.01.005
- VernacularTitle:木犀草素调控非小细胞肺癌上皮间质转化的分子机制
- Author:
Junshan RUAN
1
;
Huan ZHOU
;
Zongsheng JIANG
;
Shaoming WANG
Author Information
1. 福建医科大学省立临床医学院
- Keywords:
Luteolin;
Epithelial-mesenchymal transition;
Molecular docking;
Integrin
- From:
Herald of Medicine
2018;37(1):20-26
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the molecular mechanism of luteolin-regulated epithelial-mesenchymal transition (EMT) in non-small cell lung cancer. Methods Firstly,the potential target of luteolin was studied by molecular docking.The effect of luteolin on EMT markers was analyzed,and then molecular biology experiments were used to verify the results. Results The result of molecular docking showed that luteolin had a good docking effect on the integrin family,of which the lowest binding energy was -15.11 in docking with Integrin αIIbβ3.The result of flow cytometry showed that luteolin could down-regulate the expression of integrin induced by hypoxia. Conclusion Luteolin can regulate integrin expression,which suggested that luteolin can regulate EMT through integrin.This conclusion may provide new methods in prevention of tumor metastasis for traditional Chinese medicine.
