Effect of α-Asarone on Esophageal Eca-109 Cell Mitochondrial Apoptosis Pathway
10.3870/j.issn.1004-0781.2018.01.006
- VernacularTitle:α-细辛醚对食管癌Eca-109细胞线粒体凋亡通路的影响
- Author:
Baiyan WANG
1
;
Fangfang ZHANG
;
Qianqian HAN
;
Ruiqin LI
;
Yanqin ZHU
Author Information
1. 河南中医药大学基础医学院实验教学中心
- Keywords:
α-asarone;
Eca-109 cell mitochondria;
Carcinoma;
esophageal;
Cyt-c;
Smac;
Caspase3
- From:
Herald of Medicine
2018;37(1):27-30
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the effect of α-asarone on the expression level of Cyt-c,Smac,Caspase3 mRNA and protein in human esophageal carcinoma Eca-109 cell mitochondria. Methods The Eca-109 cells were cultured in vitro,and divided into the negative control group and the α-asarone treatment groups(final concentration:25,50,100 μg·mL-1).After 48 h,the morphological changes of Eca-109 cells were observed by fluorescence inversion microscope.The total RNA of cells were extracted by TRIzol method,the expressions of Cyt-c、Smac and Caspase3 were measured by RT-PCR and Western blotting. Results After Eca-109 cells were treated with different concentrations of α-asarone for 48 h,and obvious changes in the morphology were observed,the expressions of Cyt-c,Smac and Caspase3 genes and protein were increased significantly compared to the negative control group( P<0.05). Conclusion α-asarone can induce the human Eca-109 cells apoptosis by regulating expressions of mitochondrial apoptosis pathway correlation genes such as Cyt-c,Smac and Caspase3.
