Preparation and evaIuation of bacteriaI nano-ceIIuIose/chitosan composite tubes as potentiaI smaII-diameter vascuIar grafts
10.3969/j.issn.2095-4344.2017.34.010
- VernacularTitle:细菌纳米纤维素/壳聚糖复合管制备及其作为小径人工血管潜力的评价
- Author:
Xue LI
1
;
yu Jing TANG
;
han Lu BAO
;
Lin CHEN
;
Feng HONG
Author Information
1. 东华大学化学化工与生物工程学院
- From:
Chinese Journal of Tissue Engineering Research
2017;21(34):5467-5473
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: There are still no applicable vascular grafts for clinical replacement of the small-diameter blood vessels (< 6 mm). OBJECTIVE: To explore the possibility of bacterial nano-cellulose (BNC)/chitosan (CH) composite tubes as small-diameter artificial blood vessels. METHODS: BNC/CH tubes were fabricated by introducing chitosan (0.5%, 1%, 2%) into BNC tubes. Physicochemical properties of BNC/CH tubes were analyzed including macro-morphology, microstructure, density, water-holding capacity, burst pressure, mechanical properties, blood compatibility and cytocompatibility. RESULTS AND CONCLUSION: The results indicated that, as compared with the BNC tubes, the water permeability of BNC/CH tubes reduced, the density and water-holding capacity increased, as well as the axial and radial mechanical performance was enhanced, but ductility and elasticity of BNC/CH tubes were weakened. Regarding blood compatibility, hemolysis ratios of BNC and BNC/CH met the requirements of medical grade composites, while the plasma recalcification time showed that anticoagulation of BNC/CH tubes was slightly weaker than that of the BNC tubes. Cell compatibility experiments showed that porcine hip artery endothelial cells stably proliferated on the inner surface of both BNC and BNC/CH tubes, but the proliferation was more obvious on the BNC surface than on the BNC/CH surface.
