Technology Optimization of Hydrolyzing Aralia Saponins from Alcohol Extracts of Aralia elata
10.3969/j.issn.1005-5304.2018.01.013
- VernacularTitle:从龙牙楤木药材乙醇提取物中水解楤木皂苷工艺优选
- Author:
qin Li YAO
1
;
Ming TIAN
Author Information
1. 黑龙江中医药大学
- Keywords:
Plackett-Burman design;
central composite design response surface methodology;
orthogonal design;
Aralia elata;
oleanolic acid;
hydrolysis technology
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2018;25(1):59-63
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize process of hydrolyzed oleanolic acid from Aralia elata medicinal alcohol extract. Methods Plackett-Burman combined with CCD response surface design method (method 1) and orthogonal experimental design method (method 2) were used to optimize process of hydrolyzed oleanolic acid from Aralia elata medicinal alcohol extract. In method 1, the content of oleanolic acid was the dependent variable, and the hydrolysis time, the hydrochloric acid concentration, and the ratio of material to liquid were set as the independent variables; In method 2, the content of oleanolic acid was the dependent variable, and the hydrolysis time, hydrolysis temperature, the hydrochloric acid concentration, and the ratio of material to liquid were set as the independent variables. Design expert 8.0.6 Trial software was used to analyze the data. Results Considering the actual production situation, method 1 determined the optimum process was to add 15–18 times the amount of 10% to 12% hydrochloric acid, reflux hydrolysis 45–70 min; method 2 determined the optimum process was 15 times the amount of 10% hydrochloric acid, reflux hydrolysis 60 min. Conclusion By comparing method 1 and method 2, the optimum process was selected, and the difference of the content of oleanolic acid is not great. However, the former is more intuitive and convenient, with high precision repeatability, and predictability.
