Optimization of Extraction Process of Yinhuai Jiedu Granula by Response Surface Methodology
10.3969/j.issn.1005-5304.2018.01.014
- VernacularTitle:响应面法优选银槐解毒颗粒提取工艺
- Author:
Jiang ZHANG
1
;
Li ZHANG
;
SIYITI MUKADAISI
;
cui Fei ZHAO
Author Information
1. 新疆医科大学中医学院
- Keywords:
Yinhuai Jiedu Granula;
chlorogenic acid;
rutin;
baicalin;
response surface
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2018;25(1):64-68
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize extraction process of Yinhuai Jiedu Granula.Methods On the basis of single factor experiments, effects of soaking time, solvent dosage and extracting time on contents of chlorogenic acid and total flavonoids were investigated by response surface methodology. HPLC was used to determine contents of chlorogenie acid and total flavonoids with mobile phase of acetonitrile-0.1% phosphoric acid for gradient elution (0–20 min, 13%A; 20–30 min, 13%–18%A; 30–55 min, 18%–20%A; 55–65 min, 20%–23%A) and detection wavelengthes of 327 nm and 256 nm for chlorogenic acid and total flavonoids respectively. Results Optimal extraction process was as following: soaked 60 minutes and extracted twice with 12 times the amount of water per time of 130 minutes. Concentrations of chlorogenic acid, rutin and baicalin were 51.497, 68.872, 25.763 μg/g respectively. The average extract yield was 33.105%. Conclusion The optimized extraction process is feasible and stable,which can provide data support for the further research on Yinhuaijiedu granula.