Expression and Its Significance of Serum NF-κB and TNF-α in Children with Mycoplasma Pneumoniae Pneumonia
10.3969/j.issn.1671-7414.2017.05.023
- VernacularTitle:儿童支原体肺炎患者血清中NF-κB及TNF-α的表达及临床意义
- Author:
Yong LI
1
;
ya Qiu PAN
;
hua Shou ZHU
Author Information
1. 高邮市中医医院检验科
- Keywords:
Mycoplasma pneumoniae pneumonia;
NF-κB;
TNF-α
- From:
Journal of Modern Laboratory Medicine
2017;32(5):87-89,93
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the diagnostic value of the quantitative detection of serum NF-κB and TNF-α in children with Mycoplasma pneumoniae Pneumonia(MPP).Methods 150 MPP patients were enrolled in this study including 75 with wheeze and 75 without wheeze,and then collected the blood specimens.75 healthy children were recruited to the control group (NC group).The levels of NF-κB and TNF-α in different groups were detected by qRT-PCR.The performance of NF-κB and TNF-α were evaluated by receiver operating characteristic curves (ROC) and the area under the curve (AUC) (95 ％ CI).Results The relative expression of NF-κB and TNF-α in MPP children with wheeze were 3.09±0.15 and 2.14±0.11,respectively.They were significantly higher than NC group (t=11.03,6.987,P＜0.05).NF-κB and TNF-α in MPP children without wheeze were also significantly higher than control group (t =6.873,7.406,P＜ 0.05).The relative expression of NF-κB and TNF-α in children with wheeze were significantly higher than children without wheeze.The detection of NF-κB yielded an area under the curve of ROC of 0.826 5 (95％CI:0.772 8～0.880 2) in discriminating MPP patients from healthy group.The TNF-α was 0.818 3 (95％ CI:0.765 1～0.871 6) in discriminating MPP patients from healthy group.Conclusion The relative expression of NF-κB and TNF-α in children with MPP was significantly higher than that in NC group,NF-κB and TNF-a may prove to be non-invasive biomarkers for the auxiliary diagnosis of MPP.
