Isolation and characterization of mesenchymal stem cells from whole human umbilical cord with a single enzyme approach

VernacularTitle:用单酶消化法分离整根脐带源间充质干细胞
Author: Haihui LIU ¹ ; Saisai REN ; Yanling TAO ; Lulu LIU ; Hao ZHANG
Author Information

1. 济宁医学院
Keywords: Umbilical cord; Mesenchymal stem cells; Enzyme digestion
From: Chinese Journal of Postgraduates of Medicine 2017;40(11):965-969
CountryChina
Language:Chinese
Abstract: Objective To explore a new approach to isolate the umbilical cord-mesenchymal stem cells (UC-MSCs) from a whole umbilical cord. Methods Single enzyme was used to digest the whole umbilical cord,and passaged and cultured,draw the growth curve,cell cycle and cell wall antigen on the surface by flow cytometry. In particular inducing system, the adherent cells were induced into adipocytes and osteoblast. Results The mesenchymal stem cells (MSCs) were derived from the whole umbilical cord. The growth curve showed that the cell doubling time was (24.15 ± 0.49) h and the percentage of G1phase cells was 82.66%.The adherent cells expressed the CD93,CD105,CD44,CD29and CD73, and did not express the CD34, CD45, CD31and human leukocyte antigen DR (HLA-DR). UC-MSCs could differentiate into adipocytes and osteoblast. Conclusions Single enzyme approach is a good method to obtain UC-MSCs from whole human umbilical cord,and it provides a theoretical basis for the establishment of MSCs bank and clinical application.