Conservative epitope of human papillomavirus type 31 L2 can induce broad-spectrum neutralizing antibodies

VernacularTitle:HPV31型L2保守中和表位可诱发广谱中和抗体
Author: Ting ZHANG ¹ ; Xue CHEN ; yang Hong LIU ; Yan ZHOU ; rong Zhi WANG ; mei Xue XU
Author Information

1. 中国医学科学院基础医学研究所北京协和医学院基础学院生物物理及结构生物学系
Keywords: human papillomavirus type 31; L2 conservative neutralizing epitope; broad-spectrum neutralizing antibody
From: Basic & Clinical Medicine 2017;37(11):1552-1556
CountryChina
Language:Chinese
Abstract: Objective To detect the immunogenicity of conservative neutralizing epitope from human papilloma-virus type 31 ( HPV31) minor capsid protein L2, and analyze the neutralizing antibody spectrum of its immune sera.Methods Synthesize HPV31 L2 aa.17-40 peptide and conjugate with KLH by EDC.Immunize New Zeal-and white rabbits with HPV31 L2-KLH and Freud's Adjuvant.Analyze the neutralizing antibody titers of immune sera against HPVs from α4,α7,α9,α10 and β1 species by pseudovirus neutralization assay.Results In rabbits, HPV31 L2-KLH induced broad-spectrum neutralizing antibodies against at least 17 types of HPV, among which the neutralizing antibody titers against HPV31 are the highest, followed by that of HPV5/45/57. Conclusions The broad-spectrum neutralizing activity of the immune sera of HPV31 L2 is stimulated by conser-vative neutralizing epitope.The results lay the foundation of the development of broad-spectrum HPV vaccines based on HPV31 L2 neutralizing epitope.