Downregulation of GDF15 promotes proliferation of human glioblastoma cell line U87MG
10.3969/j.issn.1001-6325.2017.11.012
- VernacularTitle:GDF15表达下调促进人胶质母细胞瘤细胞系U87MG增殖
- Author:
ran Xin ZHANG
1
;
yan Zhao QIANG
;
Hao ZHUANG
;
Feng HAN
;
mei Yong LI
Author Information
1. 天津医科大学基础医学院药理学系
- Keywords:
glioblastoma;
GDF15;
chemotherapy resistance;
cell cycle
- From:
Basic & Clinical Medicine
2017;37(11):1557-1561
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of growth differentiation factor 15 ( GDF15 ) downregulation on cell proliferation of human glioblastoma U 87MG cells.Methods Human glioblastoma U87MG cells with stable GDF15 downregulation was used as shGDF 15 group.U87MG cells with scramble knockdown was used as scramble group . Protein expression levels of GDF 15 were determined by Western blot analysis .Growth curve and BrdU incorporation assays were used to observe cell proliferation .Protein expression levels of ERK 1/2 and p-ERK1/2 were determined by western blot analysis .CCK-8 assays were used to observe cell proliferation .Results Compared with scramble cells, GDF15 downregulation significantly promoted cell proliferation ( P<0.05 ) , increased DNA synthesis in S phage ( P<0.01 ) , enhanced activity of ERK pathway and cell tolerance to VM-26 ( P<0.05 ) .Moreover , ERK pathway inhibitor rescued the increased cell proliferation with GDF15 downregulation.Conclusions GDF15decrease DNA synthesis in S phage and cell proliferation of human glioblastoma U 87MG cells through inhibiting ERK pathway .GDF15 is a potential target of chemotherapy sensitivity in glioblastoma clinical treatment .
