Over-expression of KLF4 inhibits the proliferation and migration of K562 cell line
10.3969/j.issn.1001-6325.2017.11.015
- VernacularTitle:KLF4过表达抑制人白血病K562细胞系的增殖及迁移
- Author:
Bo LI
1
;
xian Ming TAN
;
yan Hai HU
Author Information
1. 重庆三峡中心医院 检验科
- Keywords:
KLF4;
K562 cell;
cell proliferation;
cell migration
- From:
Basic & Clinical Medicine
2017;37(11):1574-1578
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of over-expression of KLF 4 on the proliferation and migration ability of K562 cells.Methods The K562 cells with KLF4 over expression ( K562-KLF4 cells) were used as experimental group, and paralleled with the vector control ( K562-C1 cells) and blank K562 cell control.Real-time PCR was performed to detect the relative expression quantity of KLF4 mRNA of each group cells respectively;Western blot was performed to detect the level of KLF 4 protein of each group cells respectively .The cell proliferation was tested by MTS assay.The migration ability of K562 cells was detected by Transwell .Results Compared with K562-C1 cells and blank K562 cells, the relative expression of KLF4 mRNA of K562-KLF4 cells was significantly increased (P<0.05).The level of KLF4 protein of K562-KLF4 cells was also significantly increased, by 77.78%(P<0.05).The proliferation ability and migration ability of the K 562 cells with over-expressing KLF4 were inhibited sig-nificantly (P<0.05).Conclusions Over-expression of KLF4 could inhibit the proliferation and migration ability of K562 cells.
