Expressions of miR-20a-5p/miR-20b-Sp in gastric cancer cells and gastric carcinoma tissues and enriching analysis of their target genes and signal pathways
10.13602/j.cnki.jcls.2017.11.06
- VernacularTitle:miR-20a-5p/miR-20b-5p在胃癌细胞和组织中的表达及调控靶基因信号通路富集分析
- Author:
long Hai LI
1
;
hui Yao SONG
;
feng Zhao CHEN
;
Jing WANG
;
hong Yi TIAN
;
yuan Yuan WANG
;
Che CHEN
;
xia Yuan NIU
Author Information
1. 甘肃中医药大学临床医学院临检基础教研室
- Keywords:
miR-20a-5p;
miR-20b-5p;
gastric cancer
- From:
Chinese Journal of Clinical Laboratory Science
2017;35(11):822-827
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expressions of miR-20a-5p/miR-20b-5p in gastric cancer cells and gastric carcinoma tissues,analyze their target genes and enriching signal pathways using bioinformatics methods,and explore their biological behavior and function.Methods The expression levels of miR-20a-5p/miR-20b-5p in gastric cancer cells with different differentiation such as high,middle or low differentiation,normal gastric mucosa cells,gastric cancer tissues and adjacent tissues were detected by real-time fluorescent quantitative PCR,and their clinical significance was analyzed.The target genes of miR-20a-5p/miR-20b-5p were predicted using 10 softwares affiliated to mirWALK web database,and the genes supported by more than three softwares were selected as target genes.The signal pathways of target genes were enriched by online DAVID 6.7 software.Results The expression levels of miR-20a-5p/miR-20b-5p in gastric cancer cells with different differentiation were significantly higher than that in normal gastric mucosa cells (all P ＜0.05),and that in gastric cancer tissues higher than adjacent tissues (P ＜ 0.05).The up-regulated expression of miR-20b-5p was closely related to lymph node metastasis and invasion depth (all P ＜ 0.05).Bioinformatics analysis showed that the enriched target genes of miR-20a-5p/miR-20b-5p existed in multiple signaling pathways associated with cancer.Conclusion MiR-20a-5p/miR-20b-5p may be a promising biomarker of gastric cancer,which is highly expressed in gastric cancer and is related to lymph node metastasis and invasion depth.
