Association of interleukin-6 gene-174G ＞ C polymorphisman with coronary heart disease of Chinese Han population in Nanjing area
10.13602/j.cnki.jcls.2017.11.13
- VernacularTitle:IL-6-174G＞C基因多态性与南京地区汉族人群冠心病的相关性
- Author:
bang Yue WANG
1
;
xia Hong WEI
;
ye Zhi XU
;
Yan WANG
;
Kui ZHANG
Author Information
1. 南京医科大学鼓楼临床医学院
- Keywords:
interleukin-6;
coronary heart disease;
gene polymorphism
- From:
Chinese Journal of Clinical Laboratory Science
2017;35(11):853-856
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship of-174G ＞ C polymorphism of interleukin 6 (IL-6) gene promoter region upstream-with coronary heart disease (CHD) of Chinese Han population in Nanjing area.Methods The polymorphism of IL-6-174G ＞C gene was confirmed in 235 patients with CHD and 175 healthy individuals by PCR-RFLP,and Hardy-Weinberg equilibrium was tested.DNA samples were selected for sequencing to verify their genotype.The concentration of blood sugar,lipid,C reaction protein (CRP) and glycosylated hemoglobin (HbAlc) in the samples were measured simultaneously.Results The genotype distributions of GG,GC and CC were 98.7％,1.3％ and 0 in CHD group and 97.7％,2.3％ and 0 in control group,respectively.The frequencies of G and C alleles were 99.4％,0.6％ and 98.9％,1.1％ in the two groups.There were no statistical significance for frequencies of genotype and alleles between the two groups (all P ＞ 0.05).Compared with the control group,the differences of smoking,systolic blood pressure (SBP),diastohc blood pressure (DBP),triglyceride (TG),cholesterol (CHOL),low-density lipoproteins-cholesterol (LDL-C),high-density lipoproteins-cholesterol (HDL-C),apolipoprotein A-I (ApoA-I),ApoB,CRP and HbA1 c were statistically significant (all P ＜ 0.05),while age,sex and blood glucose were not statistically significant (all P ＞ 0.05).Conclusion IL-6-174 G ＞ C gene polymorphism should not remarkably correlated with CHD in Chinese Han population in Nanjing area.
