Molecular epidemiological characteristics and genotype analysis of norovirus in Zhenjiang region
10.13602/j.cnki.jcls.2017.11.14
- VernacularTitle:镇江地区诺如病毒分子流行病学特点及基因型分析
- Author:
feng Jin XU
1
;
Hong XU
;
sheng Yin SONG
;
Yuan XUE
;
he Shi SHAO
;
xing Hong SHEN
Author Information
1. 江苏大学医学院
- Keywords:
norovirus;
molecular epidemic disease;
genotype
- From:
Chinese Journal of Clinical Laboratory Science
2017;35(11):857-861
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the molecular epidemiological characteristics and genotype distribution of norovirus in Zhenjiang region during 2015 and 2016.Methods The fecal samples from diarrhea patients in Zhenjiang region were collected,and the expression level of norovirus capsid protein gene was detected by reverse transcription PCR (RT-PCR).The PCR products were validated by gene sequencing.Then,phylogenetic tree was constructed with Mega software,and sequence evolution and genotype were analyzed.Results The positive rate of norovirus infection was 5.30％ (85/1 605).Among them,the detection rates of G Ⅰ genogroup and G Ⅱ genogroup were 0.87％ (14/1 605) and 4.55％ (73/1 605),respectively,and 2 samples were detected both of G Ⅰ and G Ⅱ genogroups.The highest positive rate of G Ⅱ genogroup occurred in adolescent patients aged from 10 to 20 years (17.80％).During 2015,the detection rate of norovirus infection was the highest in January,and then in November and February.During 2016,the detection rate of norovirus infection was the highest in December,and then in March and April.The main genotypes of norovirus in 2015 and 2016 were new G Ⅱ.17 variant (63.89％) and G Ⅱ.4 Sydney_2012 strain (35 ％),respectively.Conclusion The infection of norovirus in Zhenjiang region appears G Ⅱ as main genogroup,adolescent as main patients,November to April of the next year as main epidemic seasons,and G Ⅱ.17 variant and G Ⅱ.4 Sydney_2012 strain as main epidemic genotypes.
