Study on Separation and Purification of Total Flavonoids from Polygonum hydropiper Linn. by Macroporous Resin
10.3969/j.issn.1005-5304.2018.01.016
- VernacularTitle:大孔吸附树脂分离纯化辣蓼总黄酮研究
- Author:
ling Man SONG
1
;
yu Jun TAO
;
Jian YANG
;
jun TING HU
Author Information
1. 广西大学动物科学技术学院
- Keywords:
macroporous adsorption resin;
Polygonum hydropiper Linn.;
total flavonoids;
separation and purification
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2018;25(1):74-78
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the separation and purification of total flavonoids from Polygonum hydropiper Linn. by macroporous adsorption resin; To investigate the effects of various factors in the process of static and dynamic adsorption on effects of adsorption and desorption to determine the optimum capability. Methods The total flavonoids from Polygonum hydropiper Linn. was extracted; the contents of flavonoids of ethyl acetate part (FEA) and flavonoids of n-butanol part (FNB) were detected; the adsorption experiment of FEA and FNB was conducted by macroporous adsorption resin. D101, AB-8, DM130, and XDA-8 macroporous resin were used to optimize the separation and purification process. Results The XDA-8 macroporous resin was selected to enrich total flavonoids from Polygonum hydropiper Linn. The optimum process was: adjusting the pH value of FEA flavonoids to 6, the concentration of sample was 750 g/mL, eluting with 75% ethanol to elute 5 bed volumes at a flow rate of 1 bed per hour; adjusting the pH value of FNB flavonoids to 6, the concentration of sample was 1 mg/mL, eluting with 60% ethanol to elute 5 bed volumes at a flow rate of 1 bed per hour. Conclusion XDA-8 macroporous adsorption resin is helpful to separate and purify the total flavonoids of Polygonum hydropiper Linn.by the best process.
