Role of Akt during Islet-1 inducing C3H10T1/2 cells to differentiate specifically into cardiomyocytes
- VernacularTitle:Akt在Islet-1诱导间充质干细胞C3H10T1/2向心肌细胞特异分化过程中的作用
- Author:
yu Xing LAI
1
;
Jing ZHU
;
Jie TIAN
;
Qin YI
;
Xueni CHEN
Author Information
1. 重庆医科大学附属儿童医院儿科研究所儿童发育与疾病研究教育部重点实验室儿童发育重大疾病国家国际科技合作基地儿科学重庆市重点实验室
- Keywords:
Islet (1-);
Akt;
MK-2206;
mesenchymal stem cells;
cardiomyocyte
- From:
Basic & Clinical Medicine
2018;38(1):13-19
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect and mechanism of Akt during Islet -1 inducing the mesenchymal stem cells of C3H10T 1/2 cells to differentiate specifically into cardiomyocytes.Methods Developing a model of Islet-1 over-expression cells, Lentivirus infection efficiency of cells was detected by flow cytometry detection tech-nology.Cell proliferation was tested by CCK-8.The protein expression of Islet-1, cTnT and p-Akt/T-Akt was measured by Western blot.The mRNA expression of GATA4,Nkx2.5 and Mef2c were tested by real-time PCR. Results With the increasing of MK-2206 concentration, the inhibition rate of cell proliferation increased ( P<0.05) ,and the best inhibition concentration of Akt was 8nmol /L; With prolongation of Islet-1 inducing time,the protein expression of p-Akt/TA-kt reduced ( P<0.05 );The gene expression of myocardial specific transcription factor GATA4, Nkx2.5 andMe f2c increased( P<0.05); treated with MK-2206, the geneexpres-sion of GATA4, Nkx2.5 and Mef2cincreased significantly at the first week and then reduced ( P<0.05) . Conclu isons Akt plays different effects in different differentiation stages during the Islet-1 inducing the cells into cardiacs-pecific differentiation process .
