Ang Ⅱinduces up-expression of secreted frizzled-related protein 5 in cardiomyocyte hypertrophy of rats
- VernacularTitle:AngⅡ诱导大鼠心肌细胞肥大过程中分泌型卷曲相关蛋白5表达上调
- Author:
Xin JIN
1
;
yan Bing GUO
;
jun Yong LI
Author Information
1. 河北医科大学第二医院心内科,河北石家庄050000
- Keywords:
secreted frizzled-related protein 5;
angiotensin Ⅱ;
cardiomyocyte;
hypertrophy;
JNK pathway
- From:
Basic & Clinical Medicine
2018;38(1):20-25
- CountryChina
- Language:Chinese
-
Abstract:
Obj ective To investigate the mechanism of secreted frizzled-related Protein 5 ( sFRP5) expression in cardiomyocyte hypertrophy.Methods In vivo experiment, neonatal rat ventricular myocytes were exposed to Ang Ⅱ(10-6 mmol/L, 48 h).Telmisartan, Y27632, PD98059,SB203580 and SP600125 were used to block angiotensin type 1 receptor( AT1R) , Rho/ROCK, p38 MAPK, ERK1/2 and JNK pathway, respective-ly.Western blot was applied to determine the expressions of sFRP5, ROCK1, ROCK2, total MYPT1, p-MYPT1.RT-PCR was used to determine sFRP5 expression.Results There was significant inhibition of sFRP5 expression when treated with Y37632 and SP699125, but less with SB203580 and PD98059 in AngⅡ-induced cardiomyocytes.Moreover, telmisartan down-regulated the expression of ROCK1, but no effect on the expression of ROCK2.Conclusions The expression of sFRP5 is up-regulated mainly by Rho/ROCK1/JNK pathway in cardiomyocyte hypertrophy induced by Ang Ⅱ.
