Correlation of KRAS mutations and clinicopathological parameters in colorectal carcinoma
- VernacularTitle:结直肠癌KRAS突变与临床病理特征的关系
- Author:
Long JIN
1
;
xia Yu SUI
;
ping Li WANG
;
feng Ling CHEN
;
yan Xiao CHEN
Author Information
1. 福建医科大学省立临床医学院福建省立医院病理科
- Keywords:
colorectal neoplasms;
DNA mutational analysis;
genes;
ras;
polymerase chain reaction
- From:
Basic & Clinical Medicine
2018;38(1):32-36
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate mutation rate and types of KRAS in colorectal carcinoma , and to analyze the relationship between KRAS mutation and clinicopathological parameters in patients with colorectal carcinoma ( CRC) .Methods Scorpions Amplification Refractory Mutation System ( ARMS) fluorescence quantitative PCR was performed to detect the mutations in codons 12 and 13 of KRAS and to correlate between clinicopathological characteristics and the presence of various KRAS mutations of colorectal carcinoma .Results KRAS mutations were identified in 518 patients ( 42.92%) , including G12D ( 197 cases, 16.32%) , G12V ( 125 cases, 10.36%),G12C(40 cases, 3.31%),G12S(29 cases, 2.40%),G12A(21cases, 1.74%),G12R(7 cases, 0.58%),13D(117 cases, 9.69%).Female patients had a higher KRAS mutation rate than male (46.21%, 238/515 vs.40.46%, 280/692, P<0.05 ) .KRAS mutation was significantly higher in right colon cancer (46.45%,131/282)and in rectal cancer(44.50%,255/573)than that in left colon cancer(37.50%,132/352) (P<0.05,P<0.05).Conclusions There are many types of KRAS mutations in colorectal cancer, and many mutation types exist simultaneously .The detection rate of KRAS mutation is higher in female CRC patients than in the males.The detection rate of KRAS mutation is significantly higher in right colon cancer and in rectal cancer than that in left colon cancer.
