miR-489 inhibits the stem cell phenotype and epithelial-mesenchymal transition of colon cancer by regulating the expression of TWIST 1
- VernacularTitle:miR-489通过调控TWIST1的表达抑制结肠癌干细胞表型和上皮间质转换
- Author:
Li REN
1
;
chao Ying LI
;
Kai JIA
;
Li ZHANG
;
Chen MI
Author Information
1. 西安交通大学第一附属医院消化内科
- Keywords:
miR-489;
TWIST1;
colon cancer;
cancer stem cells;
epithelial-mesenchymal transition
- From:
Basic & Clinical Medicine
2018;38(1):51-56
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects and regulation mechanism of miR-489 on the stem cell phenotype and epithelial-mesenchymal transition of colon cancer cells in vitro.Methods The miR-489 expression of colon cancer cell lines ( HT29, SW480 and SW620, HCT116) and normal intestinal epithelial cells HIEC were detec-ted by RT-qPCR, the miR-489 expression in colon cancer cells was raised by gene transfection technology, the colon cancer stem cell markers CD133, CD44, EpCAM, ALDH1 and epithelial marker E-cadherin, mesenchy-mal cells markers vimentin and N-cadherin were detected by Western blot.The expression of TWIST1 mRNA and protein were detected by RT-qPCR and Western blot.Results The miR-489 relative expression in four kinds of colon cancer cells ( HT29, SW480 and SW620, HCT116) were significantly lower than that of normal HIEC in-testinal epithelial cells, the difference was statistically significant ( P<0.05) .Up-regulation of miR-489 expres-sion in HT29 and HCT116 cells leaded to lower expression of colon cancer stem cell marker CD133, CD44, EpCAM ALDH1 and mesenchymal cells markers Vimentin , N-cadherin, higher expression of epithelial markers ;E-cadherin (P<0.05).Also, up-regulation of miR-489 expression in HT29 and HCT116 cells leaded to lower ex-pression of TWIST 1 mRNA and protein ( P<0.05 ) .Conclusions miR-489 is down-regulated in colon cancer cells and up-regulation of miR-489 expression inhibits stem cell phenotype and epithelial-mesenchymal transition through targeting TWIST1 in colon cancer .
