Influence of targeted gene VEGF silencing of bladder cancer cell line T24 on differentiation , maturation and function of dendritic cells
- VernacularTitle:靶向沉默膀胱癌T24细胞系VEGF对树突状细胞分化成熟及免疫功能的影响
- Author:
ke Yi HUANG
1
;
jun Si DIAO
;
Ping LIANG
;
ting Ting ZHOU
;
tao Zhi WEI
;
Wei YANG
;
Liang WANG
Author Information
1. 成都军区总医院泌尿外科
- Keywords:
bladder cancer;
microenvironment;
VEGF;
DC
- From:
Basic & Clinical Medicine
2018;38(1):57-62
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the influence of targeted gene VEGF silencing of bladder cancer cell line T 24 on differentiation, maturation and function of dendritic cells .Methods A lentiviral vector named LV-VEGFA-RNAi ( experimental group ) for gene silencing targeting VEGF and a lentiviral vector named LV-CON ( negative control group ) without any valid sequences were constructed .The blank control group accepted no intervention measures . The expression of VEGF's mRNA and protein of T24 cells from each group were detected by RT-PCR and ELISA respectively .Then the immature DCs were co-cultured respectively with the supernatant of all the groups as men-tioned above.CD1a, CD83 as the maturation marker and CD86 as the immunity marker of the DCs were detected by flow cytometry.Results The expression of VEGF's mRNA and protein of T24 cells in the experimental group were obviously inhibited ( P<0.05 ) as compared with that in the negative control group and the blank control group.DCs of the experimental group had an obviously increased ( P<0.05 ) expression of CD1a, CD83 and CD86 compared with the negative control group and the blank control group .Conclusions Targeted gene VEGF silencing by RNAi has advantages to the growth and immunity of DCs , which may strengthen the anti-tumor ca-pacity of the DCs by repairing their damaged immune monitoring function .
