miR-202 promotes the differentiation of 3T3-L1 preadipocyte via inhibiting PGC1β expression
- VernacularTitle:miR-202通过抑制PGC1β表达促进3T3-L1前脂肪细胞分化
- Author:
Di WU
1
;
yan Hong LING
;
Cen CAO
;
qin Jian HE
;
si Si YANG
;
fang Kai ZHANG
;
dong Shui FENG
Author Information
1. 南华大学生理学教研室
- Keywords:
miR-202;
3T3-L1 preadipocyte;
adipocyte differentiation;
PGC1β
- From:
Basic & Clinical Medicine
2018;38(1):63-68
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect and molecular mechanism of miR-202 on the differentiation of 3T3-L1 preadipocyte .Methods Through lentivirus infected with 3T3-L1 preadipocytes , we set up the AMO-miR-202 group and the random control group , then, these cells were induced to differentiate , nine days later, differentiation was assessed by Oil Red O staining and we examined the mRNA expression of PPARγ2 and aP2 by RT-PCR method. We examined the mRNA expression of PPARγ2,aP2 and PGC1βby Western blot method .Results After packa-ging lentivirus with AMO-miR-202 and random sequence control miRNA through cell line 293T, 80%-90%cells with fluorescence were found under fluorescence microscope; After these two lentivirus respectively infected with 3T3-L1 preadipocytes, About 70%-80%cells with fluorescence were found under fluorescence microscope .Oil Red O staining test showed that these cells with Oil Red O stained bright red fat droplets of AMO-miR-202 group and PPARγ2 and aP2 mRNA expression in the AMO-miR-202 group significantly lower than control groups (P<0.05). Western blot assay showed that the protein expression of PGC 1βin the AMO-miRNA-202 group was significantly increased(P<0.05), but the expression of aP 2 and PPARγ2 was significantly decreased (P<0.01).However, the random control group and the adipocyte group had no significant effect on the above indexes .Conclusions miR-202 can promote the differentiation of 3T3-L1 preadipocyte by inhibiting the protein expression of PGC 1βand im-proving the protein expression of PPARγ2 and aP2.
