Isocorydine inhibits the growth of human cervical carcinoma ( Siha ) cells xenografts in nude mice
- VernacularTitle:异紫堇碱抑制裸鼠人宫颈癌Siha细胞移植瘤的生长
- Author:
zhen Zhong LIANG
1
;
Jing ZENG
;
Li CHEN
;
Bin LIU
;
Fang WANG
Author Information
1. 兰州大学第二医院妇产科
- Keywords:
isocorydine;
Siha cells;
xenografts;
protein expression
- From:
Basic & Clinical Medicine
2018;38(1):69-73
- CountryChina
- Language:Chinese
-
Abstract:
Objective Study on the effect of isocorydine in human cervical cancer Siha cells xenografts in nude mice , to explore the inhibition mechanism of isocorydine in cervical carcinoma .Methods Establishment of human cervical cancer Siha cells subcutaneous xenografts model in BALB /c (nu/nu) nude mice.When the average diameter of the transplanted tumor≥0.5 cm, mice were randomly assigned into control group and experimental group .In experimental group, the model was administered by intraperitoneal injection of different doses of isocorydaline .After 4 weeks, the tumor tissues were removed , the histopathological changes of the tumor were observed by HE staining , and the ex-pression of proteins in the tumor tissue were observed by immunohistochemical staining and Western blot .Results Compared with the control group , the tumor volume of experimental group was significantly decreased ( P<0.05);the cell morphology transformed from stem cells to epithelioid cells , and the expression of E-cadherin was significantly in-creased while the expression of HPV 16E6 and vimentin was decreased .Conclusions Isocorydine may inhibit the de-velopment of cervical cancer by inhibiting the expression of E 6 protein and EMT-related signaling pathway .
