Vinorelbine induces apotosis and decreases telomerase activity in human epithelial ovarian cancer cells line SKOV 3
- VernacularTitle:长春瑞滨诱导人上皮性卵巢癌细胞系SKOV3凋亡及降低端粒酶活性
- Author:
yuan Li SHEN
1
;
mei Dong XU
;
han Xiao LIU
;
Mei YANG
;
ling Ya WEN
Author Information
1. 重庆市妇幼保健院妇产科
- Keywords:
epithelial ovarian cancer;
vinorelbine;
apotosis;
telomerase activity
- From:
Basic & Clinical Medicine
2018;38(1):87-90
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of different concentrations of vinorelbine on apoptosis ,telomerase ac-tivity and expression of human telomerase-reverse transcriptase gene ( hTERT ) in human epithelial ovarian cancer cells SKOV3.Methods Ovarian cancer cells SKOV 3 were treated with vinorelbine under different concentrations . The cell proliferation was measured by cell counting kit-8 ( CCK-8) assay , and the cell apoptosis was detected by flow cytometry.The telomerase activity of SKOV3 cells was determined by TRAP-PAGE-silver staining;The mRNA expression of hTERT was performed by RT-PCR assay .Results Vinorelbine could significantly inhibited the prolifer-ation of SKOV3 cells,induce cell apoptosis (P<0.01),reduce the telomerase activity and expression of hTERT mRNA (P<0.01), in dependent of a concentration-time manner.Conclusions The detection of telomerase activity and the mRNA expression of hTERT might be vital for predicting the prognosis of patients with epithelial ovarian cancer .