Up-regulation of TRPC3 in the Cortex of Rats with Kainic Acid-induced Seizures
10.3870/j.issn.1672-0741.2017.06.008
- VernacularTitle:TRPC3在海人酸诱导的癫痫发作大鼠大脑皮质中表达上调
- Author:
Min LU
1
;
Qing HE
;
Xiaoying ZHU
Author Information
1. 河南科技大学医学院人体解剖与组织胚胎学教研室
- Keywords:
epilepsy;
TRPC3;
kainic acid;
cortex
- From:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
2017;46(6):656-659,664
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression change of TRPC3 in cortex of rats with Kainic acid(KA)-induced sei-zures,and to explore the role of TRPC3 in epileptogenesis.Methods Fifty-four adult Sprague Dawley(SD)rats were randomly divided into two groups(18 for control group,36 for epilepsy group).The behavior of rats was observed and recorded.The epi-lepsy group was treated with KA(2 μg/kg,about 7 μL,lateral intracerebroventricular injection),and control group was treated with isotonic Nachloride(equivalent volume with KA,lateral intracerebroventricular injection).For epilepsy group,samples were taken 48 and 72 h after seizure burst.For control group,samples were taken 48 h after isotonic Nachloride injection.The mRNA level of TRPC3 in cortex was detected and recorded by RT-PCR,and the protein level of TRPC3 in cortex was measured by Western blotting,and immunohistochemistry was also used to display the TRPC3 expression in cortex.Results The control group showed no seizure activity,and the epilepsy group showed classical seizure activity(Ⅳ-Ⅴ level)about 5 min after injection and last for several hours.The mRNA and protein levels of TRPC3 in epilepsy group were both higher than those in control group with obvious distinction(P< 0.05).Conclusion The TRPC3 level increases in cortex of rats with KA-induced sei-zures.These results point out that TRPC3 is possibly involved in epilepsy etiopathogenesis and development.
