Recent Advances in Sensitive Detection of Kinases
10.11895/j.issn.0253-3820.171275
- VernacularTitle:激酶检测方法的最新研究进展
- Author:
Yang LIU
1
;
Ran Xiao ZOU
;
Chen Chen LI
;
Yang Chun ZHANG
Author Information
1. 山东师范大学化学化工与材料科学学院
- Keywords:
Kinases;
Sensitive detection;
Analytical methods;
Review
- From:
Chinese Journal of Analytical Chemistry
2018;46(1):11-19
- CountryChina
- Language:Chinese
-
Abstract:
Kinases are a class of enZymes that catalyZe the transfer of a phosphate group from a high-energy molecule to their substrates ( i.e., phosphorylation ).Kinase-induced intracellular phosphorylation plays important roles in a variety of cellular processes including metabolism, cell signaling, protein regulation, DNA replication and repair.Consequently, kinases have become potential biomarkers for disease diagnosis and drug discovery, and the development of simple and sensitive methods for kinase assay is highly desirable.In this review, we summariZe the recent advances in kinase assays with protein kinase A (PKA), casein kinase-2 ( CKⅡ) and T4 polynucleotide kinase ( T4 PNK) as the models.We focus on the newly emerging methods for kinase assays including fluorescent, single-molecule detection, colorimetric, chemiluminescent, bioluminescent, and electrochemical and photoelectrochemical methods.Furthermore, we give a new insight into the future direction of kinase assays.
