Obestatin attenuates apoptosis induced by high glucose in INS 1 cells
- VernacularTitle:肥胖抑制素减轻高糖致大鼠INS1细胞的凋亡
- Author:
Min XU
1
;
Wei WANG
;
xu Fang JIANG
;
Hong QIAO
;
Ling ZHANG
Author Information
1. 哈尔滨医科大学附属第二医院内分泌与代谢病科
- Keywords:
obestatin;
glucotoxicity;
INS1 cells;
protection
- From:
Basic & Clinical Medicine
2018;38(1):7-12
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of obestatin on the apoptosis of rat pancreatic islet cell line INS1 induced by high glucose .Methods INS1 cells were cultured in different concentrations of glucose .The survival rate and proliferation of INS1 cells were detected by MTT method;Hoechst33258 nuclear staining was used to de-tect nuclear morphology.caspase-3 method was used to study the relationship between the protective effect of obestatin and the PI3K pathway;Finally,using real-time PCR detection of FOXO1 and SREBP1c, Bax, PDX-1 expression, to further clarify the protective effect of obestatin on cells.Results In high glucose condition,obesta-tin promoted the proliferation of INS1 cells at 100 nmol/L,and promoted the proliferation of INS 1 cells significantly ( P<0.01 , compared with the control group and high glucose group ) .Obestatin can reduce high glucose-induced apoptosis(P<0.01).The expressions of FOXO1,SREBP1c,Bax and PDX-1 decreased,while the expression of FOXO1,SREBP1c,Bax and PDX-1 increased in high glucose group .Conclusions OB can attenuate the injury of INS1 cells induced by high glucose in rats .
