Rapid Identification of Oligosaccharide Isomers by Electron Capture Dissociation in Combination with Metal Ions
10.11895/j.issn.0253-3820.171261
- VernacularTitle:应用电子捕获解离技术结合金属离子快速识别寡糖异构体
- Author:
Jie Xue BAI
1
;
Jing Wen YU
;
Han Yi WANG
;
Lei SHI
;
Rui SU
;
Feng Rui XU
;
Zhou Da CHEN
;
Mei Hong YANG
;
Ying Shu LIU
Author Information
1. 长春医学高等专科学校药学院
- Keywords:
Fourier transform-ion cyclotron resonance-mass spectrometry;
Electron capture dissociation;
Metal ion;
Oligosaccharide isomer
- From:
Chinese Journal of Analytical Chemistry
2018;46(1):55-60
- CountryChina
- Language:Chinese
-
Abstract:
Oligosaccharide isomers were distinguished by electron capture dissociation Fourier transform ion cyclotron resonance mass spectrometry ( ECD-FT-ICR-MS ) in combination with utiliZing alkali, alkaline earth, and transition metals ( Na+, Ca2+, Ba2+, Mg2+, Mn2+ and Co2+) as charge carriers in electrospray.Maltoheptaose, mannohexaose and laminarihexaose were taken as examples to investigate influence of metal ions on the extent of oligosaccharide fragmentation.The same types of fragmentation ions ( 0,2 A and 2,4 A) were obtained for barium- and calcium-adducted maltoheptaose.Mg2+ and Mn2+ had the similar influence ( 0,2 A, 2,4 A and 2,5 A ).Three cross-ring cleavage ions ( 1,4 A, 2,4 A and 2,5 A ) were generated in the spectrum of cobalt-associated maltoheptaose.But in the case of doping Na+into maltoheptaose, only 0,2 A ion was detected.It was found that the signals in the spectra of mannohexaose and laminarihexaose were worse than that in the spectrum of maltoheptaose, probably resulting from different numbers of adducted metal ions.The isomers, mannohexaose and laminarihexaose could be distinguished by ECD-MS in conjunction with the addition of Ca2+, Mg2+ or Co2+.The addition of Ca2+ was the best choice for analysis of oligosaccharides.
