Mitochondrial damage in early stage of pressure ulcer in rats
- VernacularTitle:大鼠压疮早期的线粒体损伤
- Author:
Yan ZHOU
1
;
Qing WANG
;
Hui HAN
;
ze Zong HE
;
lan Feng WANG
;
mei Feng XING
Author Information
1. 华北理工大学护理与康复学院
- Keywords:
pressure ulcer;
ischemia/reperfusion injury;
mitochondria;
apoptosis
- From:
Basic & Clinical Medicine
2018;38(1):42-46
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mitochondrial damage and its effect in early stage of pressure ulcer in rats.Methods Forty rats were randomly divided into 5 groups(n=8), control group(Con group) rats without stress, the experimental group was treated with of 170 mmHg for 2 h and relax 0.5 h as one cycle(1C), experi-mental group was divided into 3C, 6C, 9C and 12C group.The pathological changes of the compressed muscle tissue of the rats in each group were observed by HE staining , Western blot was used to detect the expression of Bcl-2 and Bax in the compressed tissue , and the ultrastructure of muscle fibers and mitochondria were observed by transmission electron microscope .Results There were pathological damage and gradually increased in the ex-perimental groups, with the increase of compression cycle; the expression of Bcl-2 in each experimental group was significantly increased as compared with the control group(P<0.05), in the 3C group reached the peak, and then decreased; the expression of Bax was increased gradually with the increase of compression cycle ( P<0.05) , and in the 12C group reached the peak;with the increase of the compression cycle the muscle fibers of each experimental group appeared gradually increased pathological damage:disorder, dissolution and fracture, the ridge of the mitochondria disappeared, vacuolar degeneration, et al.Conclusions In the early stage of pres-sure ulcer in a rat , it brings occurred mitochondrial damage and induces apoptosis .
