Decreased expression of surfactant protein C in lung tissue of chronic obstructive pulmonary disease rats
- VernacularTitle:慢性阻塞性肺疾病大鼠肺组织中肺表面活性物质蛋白-C表达下降
- Author:
Wei ZHANG
1
;
xia Yu WANG
;
zhen Ren ZHU
;
li Ming JI
Author Information
1. 新乡医学院基础医学院
- Keywords:
chronic obstructive pulmonary disease;
surfactant protein C;
respiratory function
- From:
Basic & Clinical Medicine
2018;38(1):47-50
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the role of surfactant protein C ( SP-C) in rat lung of chronic obstructive pulmonary disease (COPD).Methods Forty healthy conventional Sprague-Dawley (SD) rats were randomly divided into four groups , normal control group ( control group ) , smoke exposure group ( smoking group ) , lipopolysaccharide group (LPS group), smoke exposure +Lipopolysaccharide group (COPD group).The arterial partial pressure oxygen (PaO2) and arterial partial pressure of carbon dioxide pathological (PaCO2) were detected.The ultrastructure of lung tissue was observed by transmission electron microscope .Enzyme-linked immuno sorbent assay (ELISA) were performed to determine protein expression of SP-C in lung and bronchoalveolar lavage fluid ( BALF).RT-qPCR were performed to determine mRNA expression of SP-C in lung.Results Compared with control group , smoking group and LPS group, the PaO2 of COPD group was obviously lower , the PaCO2 of COPD group was obviously higher;the ultrastructure and histological analysis of lung tissues showed chronic inflammatory injury ; Compared with control group , the expression of SP-C protein in was reduced , as well as SP-C mRNA expression .Conclusions The expression of SP-C in lung of rats COPD model is down-regulated.SP-C may be involved in COPD .
