Preparation of Melamine Molecularly Imprinted Surface Plasmon Resonance Sensor and Its Analytical Application
10.11895/j.issn.0253-3820.170003
- VernacularTitle:三聚氰胺分子印迹表面等离子体共振传感器的制备及分析应用
- Author:
Li Li ZHANG
1
;
Jie LI
;
Bao Chang CHEN
;
Hua Shu ZHU
;
Jie ZHOU
;
Peng Peng SHANG
Author Information
1. 山东农业大学化学与材料科学学院
- Keywords:
Melamine;
Molecular imprinting;
Surface plasmon resonance sensor
- From:
Chinese Journal of Analytical Chemistry
2018;46(1):88-93
- CountryChina
- Language:Chinese
-
Abstract:
The melamine molecular imprinted polymer ( MIP ) membrane immobiliZed on the surface of the surface plasmon resonance ( SPR) chip was prepared with allylcyanurate and ethylene glycol dimethacrylate as a functional monomer and cross-linker, respectively.Based on the ratio of the intercept to the slope of Langmuir equation for imprinted polymer, the association constant ( Kass ) of imprinted sites in the imprinted film to melamine was calculated to be 1.40 × 108 L/mol.The SPR sensor showed high selectivity to the template molecule melamine, and the best response was obtained at pH=7.5.The method showed good linearity in the melamine concentration range of 0.1-10.0 nmol/L ( R=0.9991 ).Based on a signal to noise ratio of 3, the detection limits of melamine were 62.6 pmol/L and 56.4 pmol/L for the milk and milk powder, respectively.The recoveries were 91.5% for milk and 92.0% for milk powder.These results suggest that SPR sensing combined with MIP film is a promising alternative method for detection of melamine.
