Synthesis and Biological Application of A Water-Soluble Fluorescent Probe Based on Coumarin Derivatives for Detection of Ferric Ion

VernacularTitle:一种基于香豆素衍生物的铁离子水溶性荧光探针的合成及其应用
Author: Hui Zhao CHEN ¹ ; Yuan Yuan LI ; Juan HAN ; Yun WANG ; Peng Yan REN ; Tong CHEN ; Xu TANG ; Liang NI
Author Information

1. 江苏大学化学化工学院
Keywords: Fluorescent probe; Ferric ion; Water solubility; Cell imaging
From: Chinese Journal of Analytical Chemistry 2018;46(1):20-26
CountryChina
Language:Chinese
Abstract: A water-soluble fluorescent probe (7-diethyl amino-3-formaldehyde coumarin) for Fe3+ detection was designed and synthesiZed, and its structure was confirmed by 1 HNMR, 13 CNMR and MS spectra.This probe showed high emission intensity at 471 nm.With the continuous addition of Fe3+, the emission intensities at 471 nm decreased gradually, and showed an excellent linearity with Fe3+in the range of 0.02-60 μmol/L, and the regression equation was I=322.56-4.86CFe3+.This probe was able to detect Fe3+ qualitatively and quantitatively with the detection limit as low as 22 nmol/L.Besides, this probe showed high sensitivity to Fe3+over other metal ions.The detection process was reversible, which could be recycled for the Fe3+detection.In terms of good optical properties and the strong fluorescence in physiological pH, the probe was successfully applied in imaging Fe3+ of living Ramos cells.