A Metabonomics Study of Cervical Cancer by Ultra Performance Liquid Chromatography Coupled with Quadrupole Orbitrap Tandem Mass Spectrometry
10.11895/j.issn.0253-3820.171412
- VernacularTitle:超高效液相色谱-四极杆-静电场轨道阱质谱联用法用于宫颈癌患者尿液代谢组学研究
- Author:
Jin LU
1
;
Xue REN
;
Liang Xiang LIU
;
Feng Ze LIU
Author Information
1. 吉林大学第一医院肿瘤中心
- Keywords:
Cervical cancer;
Ultra performance liquid chromatography coupled with quadrupole orbitrap tandem mass spectrometry;
Metabonomics;
Urine;
Biomarkers
- From:
Chinese Journal of Analytical Chemistry
2018;46(1):67-73
- CountryChina
- Language:Chinese
-
Abstract:
This work aims to analyZe and compare the potential biomarkers between cervical cancer ( CC ) patients and healthy controls (HC).The urine samples of 11 CC patients (age (45.65 ± 5.6) years) and 11 HC patients (age (45.9 ± 3.2) years) were collected.The metabolites of urine samples from CC and HC were analyZed by using ultra performance liquid chromatography coupled with quadrupole orbitrap tandem mass spectrometry ( UHPLC-Q-Orbitrap MS/MS ) , which could provide evidence for early diagnose and disease pathway.The LC-MS data of urines were analyZed by principal components analysis ( PCA) and orthogonal partial least squares discriminant analysis ( OPLS-DA) to identify the potential biomarkers.Urine samples of CC patients were successfully discirminated from those of healthy controls.A total of twelve significant metabolites were found and identified as potential biomarkers according to the established UHPLC-Q-Orbitrap MS and MS/MS method.Identification of biomarkers between CC and HC patients may play an important role in the study of mechanism of UC and its pathway.
