Construction of Tumor Tissue Array on An Open-Access Microfluidic Chip
10.11895/j.issn.0253-3820.171046
- VernacularTitle:开放式微流控芯片上的肿瘤组织微阵列构建
- Author:
Guo Dong LIN
1
;
Qiong Jin LIN
;
Wen Pei LI
;
Na YANG
;
Lao Bang XU
;
Yu Da LIU
Author Information
1. 华南理工大学附属第二医院检验医学研究室
- Keywords:
Open-access microfluidic chip;
Nanoporous membrane;
Microenvironment;
Tissue microarray;
Anti-cancer drug testing
- From:
Chinese Journal of Analytical Chemistry
2018;46(1):113-120
- CountryChina
- Language:Chinese
-
Abstract:
An open-access microfluidic chip which enabled automatic cell distribution and complex multi-step operations was developed.The microfluidic chip featured a key structure in which a nanoporous membrane was sandwiched by a cell culture chamber array layer and a corresponding media reservoir array layer.The microfluidic approach took advantage of the characteristics of the nanoporous membrane.On one side, this membrane permitted the flow of air but not liquid, thus acting as a flow-stop valve to enable automatic cell distribution.On the other side, it allowed diffusion-based media exchange and thus, mimicked the endothelial layer.In synergy with a liquid transferring platform, the open-access microfluidic system enabled complex multi-step operations involving medium exchange, drug treatment, and cell viability testing.By using this microfluidic protocol, a 10 × 10 tissue arrays was constructed in 90 s, followed by schedule-dependent drug testing.Morphological and immunohistochemical assays results indicated that the resultant tumor tissue was faithful to that in vivo.Drug testing assays showed that the microfluidic tissue array promised multi-step cell assays under biomimetic microenvironment, thus providing an advantageous tool for cell research.
