Security evaluation of exosome derived from human umbilical cord mesenchymal stem cells
10.3969/j.issn.1673-4130.2017.24.005
- VernacularTitle:脐带间质干细胞来源外泌体的安全性评估
- Author:
Jun LUO
1
;
Lijun DING
;
Qiang ZHOU
;
Yiwen HAN
;
Wenling MIAO
Author Information
1. 江苏卫生健康职业学院中心实验室
- Keywords:
huc-MSCs;
exosome;
security
- From:
International Journal of Laboratory Medicine
2017;38(24):3371-3373,3376
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the security of exosome derived from human umbilical cord mesenchymal stem cell(huc-MSC-exosome)as a kind of biological product on clinical application.Methods huc-MSC-exosome were separated by ultea centrifuge. CD9 and CD63 expression of huc-MSC-exosome were tested by Western-blot.Nanoparticle analysis was used to detect the size and concentration of huc-MSC-exosome.Hemolytic experiment,systemic anaphylaxis and blood routine test were used to verify the self-safety of huc-MSC-exosome.Results huc-MSC-exosome expressed specific markers of exosome,CD9 and CD63.Moreover,huc-MSC-exosome cannot result in hemolysis,systemic anaphylaxis reaction and abnormality of blood routine test.Conclusion Exosome derived from human umbilical cord mesenchymal stem cell has general signs of exosomes and possesses the certainly security,which may provide the experimental evidence for huc-MSC-exosome clinical application.
