Experimental systemic lupus erythematosus mouse model induced by pristane
10.7501/j.issn.1674-6376.2017.10.012
- VernacularTitle:降植烷诱导小鼠系统性红斑狼疮模型研究
- Author:
yu Wen CUI
1
;
hua Mao QUAN
;
Yu FAN
;
Qi JIA
;
Lin GENG
;
Qiao ZHANG
Author Information
1. 哈尔滨工业大学材料科学与工程博士后流动站
- Keywords:
Systemic lupus of erythematosus;
mouse model;
pristane;
auto-antibodies
- From:
Drug Evaluation Research
2017;40(10):1428-1431
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the systemic lupus erythematosus (SLE) mouse model through pristaneip injection and validate the model comprehensively.Methods Female BALB/c mice of 6-8 weeks were randomly divided into two groups.Animals in model group were injected with 0.5 mL pristane by ip injection while in control group with 0.5 mL normal saline.Anti-systemic lupus erythematosus antibodies (anti-SLE) and anti-double strand DNA antibodies (anti-dsDNA) were checked before injection and monthly thereafter.Proteinuria was detected before injection and every month thereafter.All mice were bled to death 6 months after injection.Kidneys were excised to observe the histopathologic evidence of glomerulonephritis.Results The concentration of anti-dsDNA and anti-SLE antibody in sera was higher of model group than that of control group two months after pristane injection,and the concentration of antibody gradually increased within 6 months.At the sixth months,the protein concentration of urine in most model group mice was ++++.The histopathology and imunoflorescence of kidney sections indicated typical evidence of glomerulonephritis in model group.Conclusion The murine lupus model can be successfully established in female BALB/e mouse with a single ip injection of 0.5mL ofpristane.
