Effect of aqueous extract from Araliae Echinocaulis Radicis et Cortex containing serum on TGF-β/BMPs signaling pathway in primary osteoblasts
10.7501/j.issn.1674-6376.2017.11.004
- VernacularTitle:刺老苞根皮水提物对成骨细胞TGF-β/BMPs信号通路的影响
- Author:
yun Meng YAN
1
;
jiao Xiang YI
;
cheng Jin LI
;
yue Song WANG
;
bin Wen ZHOU
;
peng Ling PEI
Author Information
1. 中央民族大学中国少数民族传统医学研究院
- Keywords:
root bark of Araliae Echinocaulis Radicis et Cortex;
aqueous extract;
osteoblast;
TGF-β/BMPs signaling pathway
- From:
Drug Evaluation Research
2017;40(11):1541-1544
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of aqueous extract from Araliae Echinocaulis Radicis et Cortex (AEAE) on expression of TGF-β/BMPs signaling pathway in osteoblasts.Methods The fracture model of rat was established and randomly divided into model group,AEAE low,middle and high dose (3.6,1.8,0.9 g/kg) group.After ig administration for 7 d,the blood was taken from abdominal aorta and the serum was separated.The rats primary osteoblasts were cultured and qualified after alkaline phosphatase staining and alizarin red staining.The osteoblasts were cultured continuously for 48 h in animal serum of the corresponding group.The contents of BMP-2,TGF-β,Smad-1 and Smad-2 were detected by qRT-PCR and Western blotting method.Results Compared with model group,the mRNA level ofBMP-2,TGF-β and Smad-1 in AEAE low,middle and high dose group,Smad-2 mRNA level in middle and high dose group,and the protein level of BMP-2,TGF-β,Smad-1 and Smad-2 in low,middle and high dose groupwere significantly higher than those in model group (P＜0.05 and 0.01).Conclusion AEAE can promote the proliferation ofosteoblasts by up-regulating the expression of BMP-2,TGF-β,Smad-1 and Smad-2 in TGF-β/BMPs signaling pathway.
