Detection of 10 anti-rheumatic constituents illegally added in Chinese traditional medicine and health products by UPLC-MS/MS
10.7501/j.issn.1674-6376.2017.11.009
- VernacularTitle:UPLC-MS/MS法测定抗风湿中成药及保健品中非法添加10种抗风湿性化学成分
- Author:
shun Ting LUO
1
;
lan Gui SHI
;
yong Jian HU
;
hong Ze LIU
;
Gang SUN
;
Jing YANG
Author Information
1. 大理州食品药品检验所
- Keywords:
UPLC-MS/MS;
anti-rheumatism;
Chinese traditional medicine;
health products;
illegally addition
- From:
Drug Evaluation Research
2017;40(11):1576-1580
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a UPLC-MS/MS method for determination of 10 anti-rheumatic constituents illegally added in Chinese traditional medicine and health products preparation.Methods The column was ACQUITY UPLC BEHC18 (50 mm× 2.1 mm,1.7 μm),The mobile phase was acetonitrile-Ammonium acetate solution (containing 0.1％ Acetic acid) with gradient elution at a flow rate of 0.3 mL/min.The ion source was electrospray ionization (ESI),Multiple-reaction monitoring (MRM) was performed to identify and quantify 10 anti-rheumatic constituents.Results 10 linear calibration curves were obtained with r ≥ 0.996 1.The recoveries were determinated at three concentration and ranged from 92.5％ to 101.8％.The precision of the method was shown by RSD (n =5) ranged from 0.9％ to 3.1％.The ranges of limit of detection were from 0.001 5 to 0.018 μg,and quantitation were from 0.004 5 to 0.55 μg.The illegally added chemicals were detected with 10 batches of 27 batches of samples.Conclusion The method were simple,sensitivity,accurate,and can be used to detect Anti-rheumatic constituents illegally added in Chinese traditional medicine and health products.
