Optimization of extraction process of total triterpenoid from root of Rose odorata var.gigantean by orthogonal test and its inhibitory effect on contraction of isolated rat intestinal smooth muscle
10.7501/j.issn.1674-6376.2017.11.010
- VernacularTitle:正交试验结合体外抑制肠平滑肌收缩活性研究优化固公果根总三萜提取工艺
- Author:
yuan Hao SHI
1
;
lei Chun WANG
;
min Yan ZHAO
;
kai Tian XUE
;
lin Dai LIU
;
yao Xiao WEI
;
yi Shu QI
;
Liang CHAO
Author Information
1. 中国人民武装警察部队后勤学院
- Keywords:
orthogonal test;
the root of Rose odorata var.gigantean;
total triterpene;
extraction technology;
inhibitory ratio;
contraction of isolated rat intestinal smooth muscle
- From:
Drug Evaluation Research
2017;40(11):1581-1586
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the extraction technology of total triterpenoid from root of Rose odorata var.gigantean (TTROG) by orthogonal test combined with the contraction effect of TTROG on the isolated intestinal smooth muscle of rats in vitro.Methods UV spectrophotometric method was used to determine the contents of total triterpenoids in the TTROG extractive at the wavelength of 550 nm by taking ursolic acid as standard substance,and vanillin acetic acid as chromogenic reagent.The extraction rate of total triterpenoids was used as index to evaluate the technology based on single factor test,in which three factors were considered as follows:the concentration of extraction solvent,ratio of material to liquid,extraction time,and their interaction on extraction were studied by orthogonal experimental design.The inhibition effect of different extracts obtained from the optimized extraction process on the contraction of intestinal smooth muscle were recorded by tension transducer to the BL-420 biological experimental multi-channel physiological signal acquisition and processing system.The extraction process of TTROG was evaluated by the combination of biological activity and extraction rate with weighting method.Results The optimal extraction conditions of TTROG were as follows:extraction solvent 80％ ethanol,solid-liquid ratio 1∶10,extraction time for 2 h,three times and extraction temperature of 80 ℃.The optimized extraction rate could reach 42.12 mg/g.TTROG obtained using the optimized method showed significantly contraction effect on rat intestinal smooth muscle with dose effect dependence,and the effect on jejunum was the strongest,and the inhibition rate was 41.96％.Conclusion The optimized extraction technology is stable and effective with high extraction rate.TTROG showed the significant inhibitory function on contraction of isolated rat intestinal smooth muscle.
