Effect of cobra venom NGF on inducing apoptosis of LX2 cells by inhibiting Akt signaling pathway and its mechanism
10.3969/j.issn.1001-1978.2018.01.007
- VernacularTitle:眼镜蛇毒NGF通过PI3K/Akt促人肝星状细胞凋亡的机制研究
- Author:
tao Feng CAI
1
;
rong Xue ZHANG
;
Lin SUN
;
nan Xiu WANG
;
Ming LIAO
;
dong Jian BAN
;
Ying CHEN
;
Jun NONG
Author Information
1. 广西医科大学 蛇毒研究所
- Keywords:
hepatic fibrosis;
human hepatic stellate cell;
cobra venom;
nerve growth factor;
apoptosis;
PI3 K/Akt
- From:
Chinese Pharmacological Bulletin
2018;34(1):23-27
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the effect of cobra venom nerve growth factor ( NGF) on inducing the apoptosis of LX2 cells, the key cells of hepatic fibrosis , through Akt signaling pathway and its underlying mechanism .Methods CCK-8 method was used to detect the pro-liferation of LX2 cells at different concentrations of NGF and LY294002 .Flow cytometry was applied to detect the effect of NGF on the apoptosis of LX 2 cells. Western blot was used to study the effects of NGF and LY294002 respectively , and their combination on the p-Akt protein level .Results NGF could decrease the survival rate of LX2 cells, and the minimum effective concentration was 1mg· L-1; it increased the apopto-sis rate of LX2 cells within the rise of concentration un-der a certain of range and decreased the expression level of p-Akt, but it had no significant effect on the ex-pression of Akt .Conclusions NGF may promote the apoptosis of LX2 cells by inhibiting the activation of PI3K/Akt signaling pathway in a concentration-de-pendent manner .The study of the pathogenesis of liver fibrosis is significant for the clinical treatment of liver fibrosis.
