Abnormal SCF-C-kit system in IBS-D rat and the correlation between SCF-C-kit system and immune function
10.3969/j.issn.1001-1978.2018.01.015
- VernacularTitle:腹泻型肠易激综合征大鼠SCF/C-kit信号的变化及其与免疫功能的关系
- Author:
na Yu CHAI
1
;
mei Hong TANG
;
sheng Yu HUANG
;
zhen Chong QIN
Author Information
1. 郑州大学第一附属医院药学部
- Keywords:
IBS-D;
stem cell factor;
C-kit;
immune dysfunction;
three-factor method;
correlation
- From:
Chinese Pharmacological Bulletin
2018;34(1):68-72
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the change of stem cell factor-C-kit (SCF-C-kit) system in irritable bowel syn-drome with diarrhea ( IBS-D) and the correlation be-tween SCF-C-kit system and immune dysfunction . Methods Twelve neonatal rats were divided into nor-mal group and model group with six rats in each group . IBS-D rat model was established through three-factor method ( mother-son separation , acetic acid stimulation and constraint ) . Immunohistochemistry was used to observe in situ protein expressions of SCF and C-kit. qPCR was used for mRNA expressions of SCF and C-kit.Correlation between SCF-C-kit system and spleen coefficients , thymus coefficients , TNF-α, IL-8 , IL-10 was analyzed by statistics .Results Compared with normal group , positive rates of SCF and C-kit protein in model group both decreased , and so did mRNA ex-pression .Expression of SCF was negatively correlative with spleen coefficient , TNF-αexpression and IL-8 ex-pression , while positively correlative with IL-10 expres-sion.Expression of C-kit was negatively correlated with thymus coefficient , spleen coefficient and TNF-α. Conclusion SCF-C-kit system of IBS-D is abnormal, which may be related with immune dysfunction .
