Effects of Periplaneta americana extract Ento-A on immune function in immunosuppressed mice
10.3969/j.issn.1001-1978.2018.01.016
- VernacularTitle:美洲大蠊提取物Ento-A对免疫抑制小鼠免疫功能的影响
- Author:
Miao TANG
1
,
2
;
药用特种昆虫开发国家地方联合工程研究中心
;
xin Wan YU
;
qing Tao WU
;
ying Xiu MA
;
Heng LIU
;
gui Cheng ZHANG
;
Li BAI
;
Huai XIAO
Author Information
1. 大理大学 昆虫生物医药研发重点实验室
2. 药用特种昆虫开发国家地方联合工程研究中心
- Keywords:
Periplaneta americana;
immunosuppres-sion;
serum hemolysin;
phagorytosis;
T cell prolifera-tion;
peripheral blood
- From:
Chinese Pharmacological Bulletin
2018;34(1):72-76
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of Periplane-ta americana extract Ento-A on the immune function in immunosuppressed mice . Methods Immunosup-pressed mouse model was induced by intraperitoneal injection of cyclophosphamide in KM mice .To evalu-ate the effects of Ento-A on the immune function in im-munosuppressed mice , neutral red method and MTT assay were used respectively to detect the effects of En-to-A on the phagocytosis of peritoneal macrophages and T cell proliferation rate in mice; with sheep red blood cell as immunogen , the effects of Ento-A on the pro-duction of serum hemolysin were evaluated;peripheral blood was tested and immune organ index calculated . Results Compared with model control group , the high, medium and low doses of Ento-A could improve the expression of serum hemolysin in immunosup-pressed mice ( P<0.01 ) , and increase the spleen in-dex(P<0.01) and thymus index (P>0.05), signifi-cantly increased the content of WBC ( P<0.01 ) , PLT ( P<0.01 ) , HGB ( P<0.01 ) , while the contents of RBC was on the rise , with no significant difference ( P>0.05 ) in peripheral blood , significantly enhanced phagocytic function and T lymphocyte proliferative abil-ity in a dose-dependent manner ( P<0.01 ) .Conclu-sion Ento-A can enhance the immune function of im-munosuppressed mice .
