Salvianolic acid B regulates bone metabolism through Nox4 pathway in HFD mice
10.3969/j.issn.1001-1978.2018.01.017
- VernacularTitle:丹酚酸B通过调节Nox4信号通路改善高脂饮食小鼠骨代谢的实验研究
- Author:
li Li WANG
1
;
feng Ru MA
;
xia Hai LIU
;
yuan Ru ZHU
;
yue Chen LIU
;
Lin LI
;
bei Bei CHEN
;
qiang Qiang JIA
;
hua Si GAO
;
wei Dong ZHANG
Author Information
1. 北京中医药大学 中药学院
- Keywords:
salvianolic acid B;
mice;
femur;
high-fat diet;
Nox4;
osteoporosis
- From:
Chinese Pharmacological Bulletin
2018;34(1):77-81
- CountryChina
- Language:Chinese
-
Abstract:
Aim To evaluate the effects of salvianolic acid B ( Sal B ) on bone metabolism and its potential mechanism in high fat diet ( HFD) mice.Methods Thirty C57BL/6J male mice were divided into three groups with 10 mice each, namely normal , HFD and HFD+Sal B.HFD and HFD+Sal B mice were treated with HFD, and HFD+Sal B group mice were also with Sal B (125 mg· kg -1· d-1).After 12 weeks' treat-ment, femurs were harvested .The effects of Sal B on biomechanical strength were evaluated by biomechani-cal tests, and the effects of Sal B on bone microstruc-ture were evaluated by Safranin O/fast green staining and hematoxylin and eosin staining .The expression of nuclear factor-kappa B ( NF-κB)-p65 and NADPH ox-idase 4 ( Nox4 ) and cathepsin K in femurs was deter-mined by immunohistochemical staining . Results Maximum load and elastic load significantly decreased ,and the trabeculae became thinner and irregular in the femurs of HFD mice , while Sal B treatment could re-verse the descending biomechanical strength and the disorganized femurs bone micro-structures in HFD mice.In addition, the expressions of Nox4, NF-κB-p65 and cathepsin Kmarkedly increased in HFD mice , and Sal B possessed the ability to down-regulate the ex-pression of Nox4, NF-κB-p65, and cathepsin K in the femurs triggered by HFD .Conclusions Sal B treat-ment improves bone metabolism via regulating Nox 4/NF-κB/cathepsin K signaling pathway in HFD mice . The findings contribute to the understanding and exten-sion of the applications of Salvia miltiorrhiza and its constituents on osteoporosis .
